Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) clr4 Polyclonal antibody

Uniprot No.

O60016

Target Names

clr4

Alternative Names

clr4 antibody; kmt1 antibody; SPBC428.08cHistone-lysine N-methyltransferase antibody; H3 lysine-9 specific antibody; EC 2.1.1.355 antibody; Cryptic loci regulator 4 antibody; Histone H3-K9 methyltransferase antibody; H3-K9-HMTase antibody; Lysine N-methyltransferase 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) clr4 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histone methyltransferase which contributes to the establishment of heterochromatin by specifically methylating histone H3 to form H3K9me. Part of the Clr4 methyltransferase complex (ClrC). ClrC preferentially ubiquitylates H3K14 and ClrC-mediated H3 ubiquitination promotes clr4 methyltransferase activity. Clr4 functions as a reader and writer of H3K9 methylation. It sets the H3K9me mark and afterwards this H3K9me mark is recognized by the chromodomains of clr4 and swi6/HP1, which then recruit additional clr4 leading to the methylation of neighboring nucleosomes. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeres, ribosomal DNA repeats, and the silent mating-type region. Clr4 methyltransferase activity promotes the assembly of a tripartite complex composed of ClrC and complexes involved in siRNA generation. Apart from H3K9, methylates also non-histone proteins such as mlo3. Interacts with mlo3 to promote the processing of centromeric and antisense RNAs.

Gene References into Functions

These results demonstrate how a direct "read-write" mechanism involving Clr4 propagates histone modifications and allows histones to act as carriers of epigenetic information. PMID: 25831549
Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly. PMID: 23771057
Division of Labor between the Chromodomains of HP1/Swi6 and Suv39/Clr4 Is Required for Productive Heterochromatin Assembly. PMID: 23849629
self-association of Swi6/HP1 helps in binding to and recruitment of Clr4 and thereby in establishment and maintenance of heterochromatin by a concerted rather than a sequential mechanism. PMID: 21224386
findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities PMID: 21436456
Data show that overexpression of clr4(+) in clr4Deltaago1Delta cells results in some de novo H3K9me2 accumulation at centromeres. PMID: 21060862
Data show that the stretched morphology of lagging kinetochores in pcs1Delta and clr4Delta mutant cells is due to merotelic attachment. PMID: 20935472
The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation. PMID: 20705239
analysis of transcriptional targets of the histone H3 Lys9-specific methyltransferase Clr4 by mrna differential display PMID: 16264237
Clr4 abolished the inhibitory effect of histone h3 on the RanGAP activity required for the heterochromatin assembly. PMID: 16540522
The components of the Clr4 histone K3-K9 methyltransferase-Rik1-Cullin4 complex act concertedly at an early step in heterochromatin formation. PMID: 17114925
In a strain lacking the histone methyltransferase Clr4, crucial for the formation of heterochromatin, the mating-type region had a random localization in the nucleus. PMID: 17504808
analyses delineate sequential steps for the assembly of heterochromatic domains and suggest that the ability of Clr4 to both 'write' and 'read' H3K9me facilitates heterochromatin maintenance through successive cell divisions PMID: 18345014
APC and heterochromatin proteins Swi6 and Clr4 play a mutually cooperative role in heterochromatin assembly, thereby ensuring chromosomal integrity, inheritance, and segregation during mitosis and meiosis. PMID: 19117951

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Chromosome.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily

Database Links

KEGG: spo:SPBC428.08c

STRING: 4896.SPBC428.08c.1

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clr4 Antibody

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