CBL4 Antibody

Datasheet

Code	CSB-PA530527XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CBL4 Polyclonal antibody

Uniprot No.

O81223

Target Names

CBL4

Alternative Names

CBL4 antibody; SOS3 antibody; At5g24270 antibody; MOP9.8Calcineurin B-like protein 4 antibody; Protein SALT OVERLY SENSITIVE 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CBL4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation.

Gene References into Functions

SOS3 mediated actin filament reorganization plays important roles in plant responses to salt stress. PMID: 23052592
These data uncover a novel role of the SOS3 gene in modulation of lateral root developmental plasticity and adaptation in response to low salt stress. PMID: 21087263
CBL4 is the barley homologue of the Arabidopsis SOS3 controlling the Na+ transport. PMID: 21047983
purification and X ray structure PMID: 15213389
calcium binding is responsible for the dimerization of SOS3 PMID: 15644219

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Subcellular Location

Cell membrane; Lipid-anchor. Cytoplasm. Nucleus. Note=The cell membrane localization is S-acylation dependent.

Protein Families

Calcineurin regulatory subunit family

Database Links

KEGG: ath:AT5G24270

STRING: 3702.AT5G24270.1

UniGene: At.20610

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CBL4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background