colD Antibody

Code CSB-PA900703XA01EOL
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O55:H7 (strain CB9615 / EPEC) colD Polyclonal antibody
Uniprot No.
Target Names
colD
Alternative Names
colD antibody; wbdK antibody; G2583_2551GDP-4-keto-6-deoxy-D-mannose 3-dehydratase antibody; EC 4.2.1.168 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O55:H7 (strain CB9615 / EPEC)
Immunogen
Recombinant Escherichia coli O55:H7 (strain CB9615 / EPEC) colD protein
Immunogen Species
Escherichia coli O55:H7 (strain CB9615 / EPEC)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Involved in the biosynthesis of L-colitose, a 3,6-dideoxyhexose present in the O-antigen region of lipopolysaccharides (LPS), where it serves as an antigenic determinant and is vital for bacterial defense and survival. Catalyzes the removal of the C3'-hydroxyl group from GDP-4-keto-6-deoxy-D-mannose via a combined transamination-deoxygenation reaction. The catalysis is initiated by a transamination step in which pyridoxal 5'-phosphate (PLP) is converted to pyridoxamine 5'-phosphate (PMP) in the presence of L-glutamate. This coenzyme then forms a Schiff base with GDP-4-keto-6-deoxy-D-mannose and the resulting adduct undergoes a PMP-mediated beta-dehydration reaction to give a sugar enamine intermediate, which after tautomerization and hydrolysis to release ammonia yields GDP-4-keto-3,6-dideoxy-D-mannose as a product. In vitro, is able to catalyze the formation of GDP-4-keto-3,6-dideoxymannose using GDP-perosamine rather than GDP-4-keto-6-deoxymannose as a substrate, with no need of glutamate.
Subcellular Location
Cell membrane; Single-pass membrane protein.
Protein Families
DegT/DnrJ/EryC1 family
Database Links
CUSABIO guaranteed quality
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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