CP12-1 Antibody

Datasheet

Code	CSB-PA444308XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CP12-1 Polyclonal antibody

Uniprot No.

O22914

Target Names

CP12-1

Alternative Names

CP12-1 antibody; At2g47400 antibody; T8I13.24Calvin cycle protein CP12-1 antibody; chloroplastic antibody; CP12 domain-containing protein 1 antibody; Chloroplast protein 12-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CP12-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as a linker essential in the assembly of a core complex of PRK/GAPDH. Coordinates the reversible inactivation of chloroplast enzymes GAPDH and PRK during darkness in photosynthetic tissues.

Gene References into Functions

Single cp12-1, cp12-2, and cp12-3 mutants do not develop a severe photosynthetic or growth phenotype. In contrast, reductions of both CP12-1 and CP12-2 transcripts lead to reductions in photosynthetic capacity and to slower growth and reduced seed yield. No clear phenotype for CP12-3 was evident. PMID: 28430985
CP12 in oxygen phototrophs needs to be extended beyond light/dark regulation, and include protection of enzymes belonging to Calvine-Benson cycle from oxidative stress. PMID: 24211189
All CP12 isoforms formed, in vitro, a supramolecular complex with GAPDH and PRK, with comparable inhibitory effects on both enzyme activities. PMID: 20399532
CP12-1 and CP12-2 expression was detected in the sepals and the style of the flower, while CP12-3 was restricted to the stigma and anthers. PMID: 18974062

Subcellular Location

Plastid, chloroplast.

Protein Families

CP12 family

Tissue Specificity

Mostly expressed in flowers, hypocotyl, cotyledons, leaves, stems, and flower stalks. Barely detectable in roots and siliques. Present in root tips and lateral roots. Accumulates in the cotyledons of etiolated seedlings.

Database Links

KEGG: ath:AT2G47400

STRING: 3702.AT2G47400.1

UniGene: At.20118

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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