Product Details

Full Product Name

Rabbit anti-Oryctolagus cuniculus (Rabbit) CYP1A2 Polyclonal antibody

Uniprot No.

P00187

Target Names

CYP1A2

Alternative Names

CYP1A2Cytochrome P450 1A2 antibody; EC 1.14.14.1 antibody; CYPIA2 antibody; Cholesterol 25-hydroxylase antibody; Cytochrome P450 isozyme 4 antibody; Cytochrome P450 LM4 antibody; Cytochrome P450-PM4 antibody; Hydroperoxy icosatetraenoate dehydratase antibody; EC 4.2.1.152 antibody

Raised in

Rabbit

Species Reactivity

Oryctolagus cuniculus (Rabbit)

Immunogen

Recombinant Oryctolagus cuniculus (Rabbit) CYP1A2 protein

Immunogen Species

Oryctolagus cuniculus (Rabbit)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.

Gene References into Functions

CYP1A1 and CYP1A2 localization into different lipid microdomains is governed by their N-terminal and internal protein regions PMID: 26468279
Removal of cholesterol (an important constituent of ordered domains) led to the relocation of CYP1A2, CYP2B4 and CPR to the disordered regions. PMID: 25236845
Oxidation kinetics of toluene in CYP1A2-CYP2B4 mixtures with and without cytochrome p450 reductase (CPR) are consistent with a model in which CYP1A2 has a higher affinity for CPR binding. PMID: 23675771

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Protein Families

Cytochrome P450 family

Database Links

KEGG: ocu:100328937

STRING: 9986.ENSOCUP00000008665

UniGene: Ocu.1943

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA006396XA01RB
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

CYP1A2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background