Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPL1 Polyclonal antibody

Uniprot No.

Q5YDB6

Target Names

CPL1

Alternative Names

CPL1 antibody; FRY2 antibody; JOE1 antibody; SHI4 antibody; At4g21670 antibody; F17L22.130 antibody; RNA polymerase II C-terminal domain phosphatase-like 1 antibody; FCP-like 1 antibody; EC 3.1.3.16 antibody; Carboxyl-terminal phosphatase-like 1 antibody; AtCPL1 antibody; CTD phosphatase-like 1 antibody; Protein FIERY 2 antibody; Protein JASMONATE OVEREXPRESSING 1 antibody; Protein SHINY 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CPL1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding. Forms a complex with RCF3 that modulates co-transcriptional processes such as mRNA capping and polyadenylation, and functions to repress stress-inducible gene expression. Dephosphorylates RCF3. Involved in the dephosphorylation of EIF4A3. This dephosphorylation retains EIF4A3 in the nucleus and limits its accumulation in the cytoplasm. Is essential for the degradation of the nonsense-mediated mRNA decay (NMD) transcripts.

Gene References into Functions

These results indicate that the coding region of Luciferase is responsible for the cpl1-specific Luciferase overexpression uncoupled with the expression of the endogenous counterpart. PMID: 28692335
A subset of alternatively spliced transcripts and 5'-extended mRNAs with Nonsense-mediated decay (NMD)-eliciting features accumulated in the fry2-1 mutant, cycloheximide-treated wild type, and upf3 mutant plants, indicating that FRY2 is essential for the degradation of these NMD transcripts. PMID: 26887918
RCF2 interacts with the NAC transcription factor NAC019 and that RCF2 dephosphorylates NAC019 PMID: 24415771
These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway. PMID: 24058624
The root-to-shoot translocation of cadmium (Cd) in cpl1-2 is mediated by yet uncharacterized Cd transport mechanisms. PMID: 23455022
FRY2/CPL1 may also recruit specific factors to regulate the co-transcriptional processing of certain transcripts to deal with environmental challenges. PMID: 24146632
we report the identification of a K-homology domain-containing protein named SHINY1 (SHI1) that interacts with CPL1 to modulate gene expression. PMID: 23874224
CPL1 is involved in iron stress responses. PMID: 23144187
In the miRNA processing complex, Serrate functions as a scaffold to mediate CPL1 interaction with HYL1, which needs to be dephosphorylated for optimal activity. In PMID: 23141542
The mechanisms of reading the CTD code by two classes of protein phosphatases, plant CPLs and yeast Ssu72, that specifically dephosphorylate Ser(5) in vitro are reported. PMID: 16148005

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Subcellular Location

Nucleus. Nucleus speckle.

Tissue Specificity

Expressed at very low levels in roots, leaves, stems, flowers and siliques.

Database Links

KEGG: ath:AT4G21670

STRING: 3702.AT4G21670.1

UniGene: At.2700

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