Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPSF30 Polyclonal antibody

Uniprot No.

A9LNK9

Target Names

CPSF30

Alternative Names

CPSF30 antibody; OXT6 antibody; At1g30460 antibody; F26G16.5 antibody; F26G16.630-kDa cleavage and polyadenylation specificity factor 30 antibody; EC 3.1.21.- antibody; Protein OXIDATIVE STRESS TOLERANT 6 antibody; Zinc finger CCCH domain-containing protein 11 antibody; AtC3H11 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CPSF30 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Mediates poly(A) site selection. Binds RNA in a calcium-dependent manner. Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation. Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress. Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst).

Gene References into Functions

The 65 kDa protein encoded by CPSF30-L mediates nitrate signaling in part by regulating NRT1.1 expression. PMID: 28850721
CPSF30 mutant incapable of interacting with calmodulin has reduced fertility, reduced lateral root formation, and altered sensitivities to oxidative stress. PMID: 25546057
review of role in mRNA polyadenylation and cellular signaling PMID: 25104048
CPSF30 mediates alternative polyadenylation in Arabidopsis. PMID: 23136375
the presence of a disulfide linkage in the endonuclease motif of AtCPSF30 protein PMID: 20888817
Results indicate that AtCPSF30 is a probable processing endonuclease, and that its action is coordinated through its interaction with Fip1. PMID: 17576667
results suggest that the nuclear CPSF complex in plants is a dynamic one, and that the interactions between AtCPSF30 and other CPSF subunits are different from those existing in other eukaryotes. PMID: 19573236

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

CPSF4/YTH1 family

Tissue Specificity

Expressed in seedlings, roots, leaves, siliques, stems and flowers.

Database Links

KEGG: ath:AT1G30460

STRING: 3702.AT1G30460.1

UniGene: At.40546

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CPSF30 Antibody

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