Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) cpxA Polyclonal antibody

Uniprot No.

P0AE82

Target Names

cpxA

Alternative Names

cpxA antibody; ecfB antibody; eup antibody; ssd antibody; b3911 antibody; JW3882Sensor histidine kinase CpxA antibody; EC 2.7.13.3 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) cpxA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histidine kinase member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA. Activates CpxR by phosphorylation; has autokinase, phosphotransferase and (in the presence of Mg(2+) and/or ATP or ADP) phosphatase activity. The kinase activity is inhibited by periplasmic accessory protein CpxP; proteolysis of CpxP relieves inhibition. Involved in several diverse cellular processes, including the functioning of acetohydroxyacid synthetase I, the biosynthesis of isoleucine and valine, the TraJ protein activation activity for tra gene expression in F plasmid, and the synthesis, translocation, or stability of cell envelope proteins. Activates transcription of periplasmic protease degP, probably by phosphorylating the cognate response protein CpxR; overexpression of an outer membrane lipoprotein NlpE also leads to transcription of degP via CpxRA. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation.

Gene References into Functions

The cpxA mutant was attenuated for virulence in the kidney but not in the bladder or urine. PMID: 29311237
X-ray structures of the CpxA reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. PMID: 28552574
Experimental modifications R296A and R296D of CpxA have been observed to reduce also the CpxA activity. These observations agree with the destabilization of the R296/F403 stacking, and could be the sign of the transmission of a conformational event taking place in HAMP to the auto-phosphorylation site of histidine kinase. PMID: 27124288
CpxP modulates the activity of the Cpx system by dynamic interaction with CpxA in response to specific stresses. PMID: 25207645
Segmental helical motions and dynamical asymmetry modulate CpxA autophosphorylation. PMID: 24492262
Different regions on the periplasmic domain of CpxA modulate signal transduction. PMID: 21349329
Mutations in CpxA individually increase phosphorylation of OmpR and phosphatase activity against phospho-OmpR which results in a circuit that is responsive to changes in CpxA stimulus. PMID: 21179024
plays a role in regulation of UNG (uracil-dna glycoylase) gene expression and in controlling mutation rate PMID: 15576781

Hide All

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW3882

STRING: 316385.ECDH10B_4101

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA360072XA01ENV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

cpxA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background