cra Antibody

Code CSB-PA359806XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cra Polyclonal antibody
Uniprot No.
Target Names
cra
Alternative Names
cra antibody; fruC antibody; fruR antibody; shl antibody; b0080 antibody; JW0078Catabolite repressor/activator antibody; Fructose repressor antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cra protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Global transcriptional regulator, which plays an important role in the regulation of carbon metabolism. Activates transcription of genes encoding biosynthetic and oxidative enzymes (involved in Krebs cycle, glyoxylate shunt and gluconeogenesis, such as ppsA and fbp). Represses genes involved in sugar catabolism, such as fruB, pfkA, pykF and adhE. Binds asymmetrically to the two half-sites of its operator.
Gene References into Functions
  1. Authors show that Cra regulates growth by activating expression of the crp gene. PMID: 24923415
  2. The deletion of cra and kdpE resulted in the ablation of attaching and effacing lesions. PMID: 23073764
  3. Curli biosynthesis is regulated by Cra. Cra mutants are inpaired in iofilm formation. PMID: 21559929
  4. Two lines of evidence indicate that a total of as many as 178 promoters are under the control of Cra. PMID: 21115656
  5. Identified as new member of the polyamine modulon together with RpoN and H-NS. PMID: 17220219
  6. The glucose uptake rate and the acetate production rate were increased with less acetate consumption for the cra mutant. PMID: 18648770

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Database Links

KEGG: ecj:JW0078

STRING: 316407.21321961

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