Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CSI1 Polyclonal antibody

Uniprot No.

F4IIM1

Target Names

CSI1

Alternative Names

CSI1 antibody; POM2 antibody; At2g22125 antibody; T16B14.2Protein CELLULOSE SYNTHASE INTERACTIVE 1 antibody; Protein POM-POM 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CSI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Regulator of the microtubular cytoskeleton. Microtubule-associated protein essential for the functional association of cellulase synthase (CESA) complexes (CSCs) and cortical microtubules. Promotes dynamics of CSCs in the plasma membrane. Regulates primary cell wall biosynthesis and cellulose microfibrils organization. Required for the regulation of root cell elongation/expansion. Necessary for the formation of ovules, pollen cell wall morphogenesis and pollen tube development. Involved in anther dehiscence, via dehydration-induced microtubule depolymerization and reorganization. May play a role in early gynoecial development.; Target of the cellulase synthase (CESA) complexes (CSCs) trafficking inhibitor CESTRIN, which reduces cellulose content and alters anisotropic growth of hypocotyls; CESTRIN treatment inhibits the dynamics of CSCs.

Gene References into Functions

Small CESA compartments (SmaCCs) or microtubule-associated cellulose synthase compartments (MASCs) are critical for fast recovery of CSCs to the plasma membrane after stress is relieved in Arabidopsis thaliana. This SmaCC/MASC-mediated fast recovery of CSCs is dependent on CELLULOSE SYNTHASE INTERACTIVE1 (CSI1), a protein previously known to represent the link between CSCs and cortical microtubules. PMID: 26443667
the sustained cellulose synthesis is important for salt stress tolerance in Arabidopsis. PMID: 26503768
CSI1 and the COMPANIONS OF CELLULOSE SYNTHASE proteins fulfil distinct functions during cellulose synthesis. PMID: 26829351
The Cellulose synthase complex (CSC)-associated proteins KORRIGAN1 (KOR1) and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1 (CSI1) were differentially affected by CESTRIN treatment, indicating different forms of association with the plasma membrane CSCs. PMID: 25535279
CSI1 directly mediates the interaction between cellulose synthase complexes and microtubules. PMID: 22751327
CSI1 is important for anther development by regulating microtubule stability and cell morphogenesis. PMID: 22427339
Data propose that POM2/CSI1 provides a scaffold between the cellulose synthases and cortical microtubules that guide cellulose synthesis. PMID: 22294619
CESA interactive protein 1 (CSI1) is a microtubule-associated protein that bridges CESA complexes and cortical microtubules. PMID: 22190487
CSI1 interacts with cellulose synthase (CESA) isoforms involved in primary plant cell wall synthesis. Mutations in CSI1 cause defective cell elongation in hypocotyls and roots and reduce cellulose content. [CSI-1] PMID: 20616083

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Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Endomembrane system. Cytoplasm, cytoskeleton.

Tissue Specificity

Expressed in seedlings, roots, stems, leaves, flowers and pollen. Highly expressed in floral tissues.

Database Links

KEGG: ath:AT2G22125

STRING: 3702.AT2G22125.1

UniGene: At.24341

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CSI1 Antibody

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Target Background