CKA1 Antibody

Code CSB-PA195607XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CKA1 Polyclonal antibody
Uniprot No.
Target Names
CKA1
Alternative Names
CKA1 antibody; At5g67380 antibody; K8K14.10Casein kinase II subunit alpha-1 antibody; CK II antibody; EC 2.7.11.1 antibody; Casein kinase alpha 1 antibody; AtCKA1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CKA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates casein in vitro. The alpha chain contains the catalytic site. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor GBFl, resulting in stimulation of its DNA binding activity. CK2 phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation. Acts as circadian clock component that maintains the correct period length through phosphorylation of CCA1. Required for the maintenance and control of genomic stability and chromatin structure. May act as an ectokinase that phosphorylates several extracellular proteins.
Gene References into Functions
  1. The high pleiotropy of CK2 was sustained by analysis of global transcript profiles that showed a huge number of genes affected, involved in a wide variety of cellular processes. PMID: 21739152
Subcellular Location
Nucleus. Nucleus, nucleolus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Tissue Specificity
Seems to be present in all plant organs. But seems to be less expressed than CKA2.
Database Links

KEGG: ath:AT5G67380

STRING: 3702.AT5G67380.1

UniGene: At.24323

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