CKA4 Antibody

Datasheet

Code	CSB-PA902075XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CKA4 Polyclonal antibody

Uniprot No.

O64816

Target Names

CKA4

Alternative Names

CKA4 antibody; At2g23070 antibody; F21P24.13 antibody; Casein kinase II subunit alpha-4 antibody; chloroplastic antibody; CK2-alpha4 antibody; EC 2.7.11.1 antibody; Plastid-targeted casein kinase 2 alpha antibody; cpCK2alpha antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CKA4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. Involved in the regulation of various developmental processes. Involved in the regulation of plant growth and flowering time. Involved in retrograde signaling in plant responses to abscisic acid (ABA) and heat stress. May act as an enhancing factor in abiotic stress signaling through modulation of the expression of some molecular players in retrograde signaling. Phosphorylates RuBisCo activase (RCA) at Thr-78.

Gene References into Functions

Chloroplast-targeted casein kinase 2 phosphorylates pTAC10.[chloroplast-targeted casein kinase 2] PMID: 29330702
Through functional characterization of two independent Casein kinase alpha4 RNAi lines the authors show that the fourth alpha subunit in Arabidopsis also functions redundantly in regulating ABA response, lateral root formation and flowering time.[CK2 alpha4] PMID: 26025542
Plastid casein kinase 2 is an enhancing factor in abiotic stress signaling. PMID: 24803505
Using bacterial overexpression of cpCK2, principal features of this enzyme are shown and functional determinants of its role as a transcriptional regulator in vitro has been assigned. Recombinant cpCK2 is capable of using various plant sigma transcription factors as phosphorylation substrates. [cpCK2] PMID: 22111690

Subcellular Location

Plastid, chloroplast.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily

Tissue Specificity

Expressed in root tips, lateral root primordia, cotyledons, leaf primordia, sepals, filaments, stigma, and anthers.

Database Links

KEGG: ath:AT2G23070

STRING: 3702.AT2G23070.1

UniGene: At.26849

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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