Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CSN5A Polyclonal antibody

Uniprot No.

Q8LAZ7

Target Names

CSN5A

Alternative Names

CSN5A antibody; AJH1 antibody; CSN5B antibody; At1g22920 antibody; F19G10.12COP9 signalosome complex subunit 5a antibody; Signalosome subunit 5a antibody; EC 3.4.-.- antibody; Jun activation domain-binding homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CSN5A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Involved in CSN's deneddylation/derubylation activity. Required for the deneddylation of all cullins. Essential for the structural integrity of the CSN holocomplex.

Gene References into Functions

These findings indicate that the rescue of csn5a-2 resulted from a trans T-DNA-mediated epigenetic effect on the csn5a-2 intronic T-DNA. PMID: 26259174
The organization of a CSN5-containing subcomplex of the COP9 signalosome. PMID: 23086934
unequal role that CSN(CSN5A) and CSN(CSN5B) play in controlling the cellular derubylation of cullins PMID: 15486099
The cop/det/fus mutant phenotype may be the result of the defects caused by impaired CSN5-dependent deneddylation of cullin-containing E3s. PMID: 15923347

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase M67A family, CSN5 subfamily

Tissue Specificity

Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.

Database Links

KEGG: ath:AT1G22920

STRING: 3702.AT1G22920.1

UniGene: At.10441

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CSN5A Antibody

Product Details

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Target Background