CTF18 Antibody

Code CSB-PA343448XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CTF18 Polyclonal antibody
Uniprot No.
Target Names
CTF18
Alternative Names
CTF18 antibody; CHL12 antibody; YMR078C antibody; YM9582.03CChromosome transmission fidelity protein 18 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CTF18 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.
Gene References into Functions
  1. These results indicate that the Ctf18-RFC/Polepsilon interaction plays a crucial role in maintaining genome stability in budding yeast, probably through recruitment of this PCNA loader to the replication fork. PMID: 26987677
  2. The findings indicate that the association of Ctf18-RFC with Pol at defective replication forks is a key step in activation of the S-phase checkpoint. PMID: 26250113
  3. Ctf18 confers protection against stalling or breaks at the replication fork in cells that either lack, or are compromised for, telomerase activity, which is likely to contribute another level to telomere size homeostasis. PMID: 24533124
  4. Ctf18 RFC-like complex modifies telomeric chromatin to make it competent for normal localization to the nuclear periphery. PMID: 16525505
  5. During chromatid cohesion establishment, Ctf18 is found at the DNA replication fork. PMID: 16962805

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Subcellular Location
Nucleus.
Protein Families
Activator 1 small subunits family, CTF18 subfamily
Database Links

KEGG: sce:YMR078C

STRING: 4932.YMR078C

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