Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHTF18 Polyclonal antibody

Uniprot No.

Q8WVB6

Target Names

CHTF18

Alternative Names

6030457M03Rik antibody; C16orf41 antibody; C321D2.2 antibody; C321D2.3 antibody; C321D2.4 antibody; CHL12 antibody; chromosome transmission fidelity factor 18 homolog (S. cerevisiae) antibody; Chromosome transmission fidelity protein 18 homolog antibody; chtf18 antibody; Ctf18 antibody; CTF18_HUMAN antibody; hCTF18 antibody; MGC38008 antibody; RUVBL antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) CHTF18 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage.

Gene References into Functions

Human CTF18-RFC clamp-loader complexed with non-synthesising POLE efficiently loads the PCNA sliding clamp. PMID: 28199690
CTF18 forms a complex with RPA when replication stress is elicited by hydroxyurea treatment or UV exposure during S phase. The interaction kinetics between CTF18 and RPA is positively associated with the phosphorylation status of Chk1. PMID: 27175616
This is the first report of somatic mutations within ESCO1 and CHTF18 in endometrial tumors and of MRE11A mutations in microsatellite-stable endometrial tumors. PMID: 23755103
identification as proliferating cell nuclear antigen-binding protein PMID: 12171929
Ctf18-RFC interacts physically with pol eta, which allows DNA replication forks to overcome interference by various template structures, including damaged DNA and DNA-protein complexes that maintain chromosome cohesion PMID: 17545166
hChlR1 has a role in the establishment of sister chromatid cohesion, with Ctf18-RFC and Fen1 PMID: 18499658

Hide All

Subcellular Location

Nucleus. Note=Associates with chromatin during S phase.

Protein Families

Activator 1 small subunits family, CTF18 subfamily

Database Links

HGNC: 18435

OMIM: 613201

KEGG: hsa:63922

STRING: 9606.ENSP00000262315

UniGene: Hs.153850

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CHTF18 Antibody

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