Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans cul-2 Polyclonal antibody

Uniprot No.

Q17390

Target Names

cul-2

Alternative Names

cul-2 antibody; ccd-3 antibody; div-3 antibody; ZK520.4Cullin-2 antibody; CUL-2 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans cul-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Core component of multiple cullin-RING-based CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the CBC complex depends on the variable substrate recognition component. May function in ubiquitin-mediated degradation of CKIs to target cki-1 for degradation. CBC(zif-1) may ensure germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. As part of the CBC(fem-1) complex directs ubiquitination of tra-1. As part of the CBC(lrr-1) complex, required for the ubiquitination and dissasembly of the CMG helicase complex from chromatin at the end of DNA replication. Positive cell-cycle regulator that is required at two distinct points in the cell cycle; the G1-to-S-phase transition and mitosis. Also required for proper cytoskeletal movement and mitotic chromosome condensation.

Gene References into Functions

CUL-2 RING E3 ubiquitin-ligase acts at multiple levels to control germline development; CUL-2 may similarly regulate germline development in other organisms as well. PMID: 23555289
segregation of the germ plasm involves both stabilization of germline proteins in the germ line and cullin-dependent degradation in the soma PMID: 12894212
Fem-1 protein is a substrate-specific adaptor for a CUL-2-based ubiquitin ligase. PMID: 18502413
MEL-26 levels are kept low by the action of another type of ubiquitin ligase, which contains CUL-2 PMID: 19361490

Subcellular Location

Cytoplasm. Nucleus. Note=Nuclear in oocytes.

Protein Families

Cullin family

Tissue Specificity

In adults, highly expressed in meiotic cells and oocytes. In larvae, expressed in many proliferating cell types: P cells during the L1 stage; seam cells when they divide at every molt; vulval and somatic gonad cells in late L3 and L4 stages; and intestina

Database Links

KEGG: cel:CELE_ZK520.4

STRING: 6239.ZK520.4a

UniGene: Cel.21341

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cul-2 Antibody

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