CUL3A Antibody

Code CSB-PA288847XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CUL3A Polyclonal antibody
Uniprot No.
Target Names
CUL3A
Alternative Names
CUL3A antibody; CUL3 antibody; At1g26830 antibody; T2P11.2Cullin-3A antibody; AtCUL3a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CUL3A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the cullin-RING ubiquitin ligases (CRL), or CUL3-RBX1-BTB protein E3 ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the CRL complex depends on the BTB domain-containing protein as the susbstrate recognition component. Involved in embryo pattern formation and endosperm development. Required for the normal division and organization of the root stem cells and columella root cap cells. Regulates primary root growth by an unknown pathway, but in an ethylene-dependent manner. Functions in distal root patterning, by an ethylene-independent mechanism. Functionally redundant with CUL3B.
Gene References into Functions
  1. AtCUL3a and AtCUL3b can assemble in Arabidopsis with BTB/POZ-MATH and AtRBX1 proteins to form functional E3 ligases. [AtCUL3a] PMID: 15618422
  2. CUL3A is ubiquitously expressed in plants and is able to interact with the ring-finger protein RBX1. [CUL3A] PMID: 15659098
  3. Arabidopsis CUL3A gene is essential for normal embryogenesis. PMID: 16045478
  4. work highlights that CUL3 is essential for the normal division and organisation of the root stem cell niche and columella root cap cells PMID: 19132085
  5. CUL3a and CUL3b are essential for plant development [CUL3a] PMID: 15749712

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Protein Families
Cullin family
Database Links

KEGG: ath:AT1G26830

STRING: 3702.AT1G26830.1

UniGene: At.27879

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