CUL4 Antibody

Datasheet

Code	CSB-PA843061XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CUL4 Polyclonal antibody

Uniprot No.

Q8LGH4

Target Names

CUL4

Alternative Names

CUL4 antibody; At5g46210 antibody; MDE13.3Cullin-4 antibody; AtCUL4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CUL4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Component of the CUL4-RBX1-CDD (COP10-DDB1a-DET1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Participates in the CDD complex to light-mediated control of development. May repress photomorphogenesis through enhancing COP1 E3 ubiquitin-protein ligase activity. Acts together with the CUL4-DDB1-COP1-SPA E3 ubiquitin-protein ligase complexes in the repression of photomorphogenesis and flowering time. Component ot the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex which mediates ubiquitination and subsequent degradation of AKIN10. Component of the CUL4-RBX1-DDB1-DWA1/DWA2 E3 ubiquitin-protein ligase complex that acts as negative regulator in abscisic acid (ABA) signaling and may target ABI5 for degradation.

Gene References into Functions

RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in trichomes. PMID: 20696906
CUL4-DDB1 and COP1-SPA complexes act together to regulate photomorphogenesis. CUL4 regulates flowering time under short day conditions. PMID: 20061554
Arabidopsis CULLIN4 (CUL4) associates with the CDD complex and a common catalytic subunit to form an active E3 ubiquitin ligase both in vivo and in vitro. PMID: 16844902

Subcellular Location

Nucleus.

Protein Families

Cullin family

Tissue Specificity

Ubiquitous.

Database Links

KEGG: ath:AT5G46210

STRING: 3702.AT5G46210.1

UniGene: At.20972

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Protein Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CUL4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background