cup Antibody

Datasheet

Code	CSB-PA895165XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) cup Polyclonal antibody

Uniprot No.

Q9VMA3

Target Names

cup

Alternative Names

cup antibody; fs(2)cup antibody; CG11181 antibody; Protein cup antibody; Oskar ribonucleoprotein complex 147 kDa subunit antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) cup protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Adapter protein that plays a central role in localization of transcripts in the oocyte and in young embryos. Maintains RNA targets in a repressed state by promoting their deadenylation and protects deadenylated mRNAs from further degradation. Binds to and recruits eIF-4E to the 3'-UTR of some mRNA targets which prevents interaction between eIF4E1 and eIF4G. This may contribute to translational repression but does not appear to be necessary for it to occur. Can promote translational repression independently of deadenylation and eIF4E1 binding. Required for correct localization of eIF4E1 in the developing oocyte. Required for translational repression of oskar (osk) mRNA. Also required for the translational repression of nanos (nos) mRNA. Promotes the accumulation of the germ plasm components osk, vas and stau at the posterior pole of the oocyte and is required for germ cell development. Represses orb positive autoregulatory activity which prevents premature activation of orb and ensures its accumulation specifically in the developing oocyte. In 0-1 hour embryos, forms a complex with me31B, cup, tral and pAbp which binds to various mRNAs including maternal mRNAs, and downregulates their expression during the maternal-to-zygotic transition.

Gene References into Functions

loss of cup disrupts the localization of both the oskar mRNA and its associated proteins to the posterior pole of the developing oocyte. PMID: 27554167
cup mutants have increased numbers of satellite boutons and EPSP. cup genetically interacts with EndoA and Dap160. PMID: 26102195
Cup promotes the accumulation of the germ plasm determinants at the posterior pole of the oocyte. PMID: 22454519
The structure provides insight into the mechanism by which Cup disrupts eIF4E-eIF4G interaction and has broader implications for understanding the role of 4E-BPs in translational regulation. PMID: 22832024
Cup blocks the precocious activation of the orb autoregulatory loop PMID: 22164257
n addition to inducing translational repression, CUP maintains mRNA targets in a repressed state by promoting their deadenylation and protects deadenylated mRNAs from further degradation PMID: 21937713
Cup is an eIF4E-binding protein that functions in smg-mediated translational repression. PMID: 14685270
Cup is a translational repressor of oskar that is required to assemble the oskar mRNA localization machinery. We propose that Cup coordinates localization with translation PMID: 14691132
Cup is required to repress precocious osk translation. Cup binds the 5'-cap binding translation initiation factor eIF4E through a sequence conserved among eIF4E binding proteins. PMID: 14723848
crucial role for the Cup-eukaryotic translation initiation factor 4E (eIF4E) complex in ovary-specific developmental programs. PMID: 15465908
an important downstream effector of a Drosophila forkhead transcription factor phenotype, and regulation of translation by eIF4E is vital during environmental stress PMID: 16055649
This review describes Cup as a multifunctional protein directly and/or indirectly affecting the activity of different mRNAs and proteins by diverse molecular mechanisms in order to achieve a broad range of biological functions. PMID: 17114932
cup, nup154 double mutants exhibit much stronger oogenesis defects than single mutants PMID: 17277377
Sqd, cup, and PABP55B function together to regulate gurken translation. PMID: 18082158
Transcription of comets and cups occurs just before protamines can be detected in spermatid nuclei. PMID: 18482002
Hsp90 interacts in vitro with the translational repressor Cup. PMID: 19101615

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Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, Cytoplasmic ribonucleoprotein granule.

Tissue Specificity

Predominantly expressed in ovaries and in 0-2 hours old embryos. Weakly expressed in testis. Expressed in young embryos through stage 9, then it decreases throughout the rest of embryogenesis. In ovaries, it is expressed in germ cells throughout pre-vitel

Database Links

KEGG: dme:Dmel_CG11181

STRING: 7227.FBpp0288761

UniGene: Dm.333

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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cup Antibody

Product Details

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Customer Reviews and Q&A

Target Background