CYC8 Antibody

Code CSB-PA321324XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CYC8 Polyclonal antibody
Uniprot No.
Target Names
CYC8
Alternative Names
CYC8 antibody; CRT8 antibody; SSN6 antibody; YBR112C antibody; YBR0908General transcriptional corepressor CYC8 antibody; Glucose repression mediator protein CYC8 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CYC8 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.
Gene References into Functions
  1. Dbp2 integrates nutrient availability with energy homeostasis by maintaining repression of glucose-repressed, Cyc8-targeted genes across the genome. PMID: 28500049
  2. Mutation in CYC8 leads to increased expression levels of the hexose transporters causing a higher transport of d-xylose into the cell in the presence of d-glucose. PMID: 28363963
  3. mutations sin3 and ssn6 turned out as synthetically lethal PMID: 21104417
  4. Ssn6-Tup1 requires the ISW2 complex to position nucleosomes. PMID: 15116071
  5. Cyc8p was identified as a coactivator for Gcn4p, a transcriptional activator of amino acid biosynthetic genes. PMID: 16314536
  6. A possible implication of the structural flexibility of Ssn6 TPR1 in Tup1 recognition is discussed and a novel mode of interaction is proposed for this particular TPR-mediated complex PMID: 17634984
  7. A new prion formed by the Cyc8 (Ssn6) protein of Saccharomyces cerevisiae, is described. PMID: 19219034

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Subcellular Location
Nucleus.
Protein Families
CYC8/SSN6 family
Database Links

KEGG: sce:YBR112C

STRING: 4932.YBR112C

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