CYK3 Antibody

Code CSB-PA135068XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CYK3 Polyclonal antibody
Uniprot No.
Target Names
CYK3
Alternative Names
CYK3 antibody; YDL117WCytokinesis protein 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CYK3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in cytokinesis by recruiting INN1 to the bud neck. Cooperates with INN1 to stimulate the synthesis of the primary septum (PS) by CHS2.
Gene References into Functions
  1. Dephosphorylation of Inn1 by Cdc14 stabilizes the interaction between Inn1 and Cyk3. PMID: 22454527
  2. Data show that the Inn1 C-terminal region is necessary for localization, and distinct PXXP motifs in this region mediate functionally important interactions with SH3 domains in the cytokinesis proteins Hof1 and Cyk3. PMID: 19528296
  3. Cyk3 is part of an actomyosin ring independent cytokinesis pathway, which acts as a rescue mechanism to recruit Inn1 to the bud neck. PMID: 19707790
Subcellular Location
Cytoplasm. Bud neck. Note=Found in association with the actin ring and the cortex at the mother-bud neck.
Protein Families
CYK3 family
Database Links

KEGG: sce:YDL117W

STRING: 4932.YDL117W

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