cyoA Antibody

Datasheet

Code	CSB-PA364657XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) cyoA Polyclonal antibody

Uniprot No.

P0ABJ1

Target Names

cyoA

Alternative Names

cyoA; b0432; JW0422; Cytochrome bo(3 ubiquinol oxidase subunit 2; Cytochrome b562-o complex subunit II; Cytochrome o ubiquinol oxidase subunit 2; Cytochrome o subunit 2; Oxidase bo(3 subunit 2; Ubiquinol oxidase chain B; Ubiquinol oxidase polypeptide II; Ubiquinol oxidase subunit 2

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) cyoA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.

Gene References into Functions

Removal of three terminal cytochrome oxidase genes (cydAB, cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the Escherichia coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. PMID: 20709841
analysis of translocation of the N-tail and C-tail of the Escherichia coli inner membrane protein CyoA PMID: 16481320
Data show that the distinct domains of CyoA have different translocation requirements (YidC only and Sec/YidC) and confirms that the membrane biogenesis of subunit II of cytochrome oxidase in bacteria and mitochondria have conserved features. PMID: 16488430
CyoA is a substrate of a novel pathway that involves both SecYEG and YidC PMID: 16513637

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Cytochrome c oxidase subunit 2 family

Database Links

KEGG: ecj:JW0422

STRING: 316385.ECDH10B_0388

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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cyoA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background