cyoB Antibody

Datasheet

Code	CSB-PA359624XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) cyoB Polyclonal antibody

Uniprot No.

P0ABI8

Target Names

cyoB

Alternative Names

cyoB antibody; b0431 antibody; JW0421 antibody; Cytochrome bo(3) ubiquinol oxidase subunit 1 antibody; EC 7.1.1.3 antibody; Cytochrome b562-o complex subunit I antibody; Cytochrome o ubiquinol oxidase subunit 1 antibody; Cytochrome o subunit 1 antibody; Oxidase bo(3) subunit 1 antibody; Ubiquinol oxidase chain A antibody; Ubiquinol oxidase polypeptide I antibody; Ubiquinol oxidase subunit 1 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) cyoB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron. Protons are probably pumped via D- and K- channels found in this subunit.

Gene References into Functions

selectively 15N labeled cyt bo3 samples have led to the unambiguous assignment of Nepsilon of R71 as the nitrogen that is strongly coupled to the QH site SQ in the wild-type enzyme and gives rise to the observed 14N ESEEM signals. PMID: 22497216
Study determined the enzyme activity of cytochrome bo(3) (cbo(3))and the UQ-10 concentration in the membrane by cyclic voltammetry. PMID: 20096262
Data show that the cytochrome bo3 tightly bound ubiquinone-8 at the Q(H) site is not displaced by ubiquinol-1 even during enzyme turnover. PMID: 20416270
cytochrome bo requires Tyr288 for dioxygen reduction PMID: 15465820
FTIR analysis of redox-induced protein structural changes in cytochrome bo PMID: 16040612
analysis of heme-heme electron tunneling in the bo(3) enzyme, a bacterial relative to the mitochondrial cytochrome c oxidase PMID: 18087041

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Heme-copper respiratory oxidase family

Database Links

KEGG: ecj:JW0421

STRING: 316385.ECDH10B_0387

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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cyoB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background