cysK Antibody

Code CSB-PA359632XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cysK Polyclonal antibody
Uniprot No.
Target Names
cysK
Alternative Names
cysK antibody; cysZ antibody; b2414 antibody; JW2407Cysteine synthase A antibody; CSase A antibody; EC 2.5.1.47 antibody; O-acetylserine antibody; thiol)-lyase A antibody; OAS-TL A antibody; O-acetylserine sulfhydrylase A antibody; S-carboxymethylcysteine synthase antibody; EC 4.5.1.5 antibody; Sulfate starvation-induced protein 5 antibody; SSI5 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cysK protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
(Microbial infection) In addition to its role in cysteine synthesis, stimulates the tRNase activity of CdiA-CT from E.coli strain 536 / UPEC; stimulation does not require O-acetylserine sulfhydrylase activity. CdiA is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (experiments done in strains BW25113 and X90, both K12 derivatives). This protein is not required for CDI of strain EC93, whose toxin may function by forming inner cell membrane pores. CysK stabilizes CdiA-CT, allowing it to bind tRNA substrate; neither CdiA-CT nor CysK bind tRNA alone in vitro.
Gene References into Functions
  1. Data show that bacterial serine acetyltransferase (CysE) is activated when bound to O-acetylserine sulfhydrylase-A sulfhydrylase (CysK). PMID: 28337759
  2. The authors showed that the expression of cysK is regulated by several genetic and environmental factors in addition to CysB: two genetic factors, OmpR and CysE, and lithium. PMID: 22092684
Protein Families
Cysteine synthase/cystathionine beta-synthase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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