Product Details

Full Product Name

Rabbit anti-Rattus norvegicus (Rat) Cyp17a1 Polyclonal antibody

Uniprot No.

P11715

Target Names

Cyp17a1

Alternative Names

Cyp17a1 antibody; Cyp17Steroid 17-alpha-hydroxylase/17,20 lyase antibody; EC 1.14.14.19 antibody; 17-alpha-hydroxyprogesterone aldolase antibody; EC 1.14.14.32 antibody; CYPXVII antibody; Cytochrome P450 17A1 antibody; Cytochrome P450-C17 antibody; Cytochrome P450c17 antibody; Steroid 17-alpha-monooxygenase antibody

Raised in

Rabbit

Species Reactivity

Rattus norvegicus

Immunogen

Recombinant Rattus norvegicus Cyp17a1 protein

Immunogen Species

Rattus norvegicus (Rat)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Target Background

Function

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Gene References into Functions

Data suggest that CYP17A1 and aromatase/Cyp19a1 are induced in hippocampus by the environmental pollutant/retinoid X receptor agonist tributyltin; thus, tributyltin appears to stimulate estradiol biosynthesis. PMID: 24679120
Data from a rat model of polycystic ovary syndrome suggest that hyperandrogenism develops and this may partially up-regulate expression of steroidogenic enzymes (CYP17 and P450scc [cholesterol side-chain cleavage enzyme]) in ovarian tissue. PMID: 22798247
these data demonstrate the occurrence of P450C(17) in the deep cerebellar nuclei of the rat PMID: 22800812
Data demonstrate, for the first time, the presence and anatomical distribution of P450C(17) in the vestibular nuclei. PMID: 21388459
Dibromoacetic acid can directly target rat Leydig cells and downregulate testicular CYP17 expression with a resulting decreased testicular testosterone production. PMID: 21046364
immunolocalization of 3beta-hydroxysteroid dehydrogense, cytochrome P450c17 and androgen receptor were investigated in rat cumuli oophori of late pre-ovulatory follicles and in post-ovulatory COCs bearing fertilized oocytes PMID: 19775733
Results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions. PMID: 20356859
17beta-estradiol is synthesized by P45017alpha and P450 aromatase localized in hippocampal neurons from endogenous cholesterol PMID: 14694190
CYP17 is expressed in rat kidney throughout the life-span and has a role in autonomous hormonal steroidogenesis PMID: 15261307
Cyp17a1 in beta-cells of rat pancreas and its local steroidogenesis are reported. PMID: 18556192
GDF-9 plays an important role in promoting preantral follicle growth by up-regulating follicular androgen biosynthesis. GDF-9 is essential for CYP17A1 expression during follicular development from the preantral to the early antral stage. PMID: 19213837
The expression of the mouse CYP17A1 gene is epigenetically controlled. PMID: 19403566

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Subcellular Location

Endoplasmic reticulum membrane. Microsome membrane.

Protein Families

Cytochrome P450 family

Database Links

KEGG: rno:25146

STRING: 10116.ENSRNOP00000027160

UniGene: Rn.10172

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Cyp17a1 Antibody

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