D14 Antibody

Datasheet

Code	CSB-PA582010XA01OFG
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Oryza sativa subsp. japonica (Rice) D14 Polyclonal antibody

Uniprot No.

Q10QA5

Target Names

D14

Alternative Names

D14 antibody; D88 antibody; HTD2 antibody; Os03g0203200 antibody; LOC_Os03g10620Strigolactone esterase D14 antibody; EC 3.1.-.- antibody; Protein DWARF 14 antibody; Protein DWARF 88 antibody; Protein HIGH-TILLERING DWARF 2 antibody

Raised in

Rabbit

Species Reactivity

Oryza sativa subsp. japonica (Rice)

Immunogen

Recombinant Oryza sativa subsp. japonica (Rice) D14 protein

Immunogen Species

Oryza sativa subsp. japonica (Rice)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Strigolactone-dependent association of D14 with D3 and D53 (a repressor of SL signaling) triggers D53 ubiquitination and degradation. Hydrolyzes the butenolide ring of SLs. A reaction product D-OH is trapped in the cavity of D14, inducing the interaction with SLR1, and probably with other proteins such as D3 and D53. Contributes to the negative regulation of gibberellin signaling.

Gene References into Functions

Data suggest that Os03g0203200 (D88) gene was a candidate gene for quantitative trait locus (QTL) qPPB3. PMID: 25079308

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

AB hydrolase superfamily

Tissue Specificity

Expressed in the parenchyma cells of the root stele and lateral roots, vascular tissues of vein and leaf sheath, ligule base, auricle base and stem base.

Database Links

KEGG: osa:4331983

STRING: 39947.LOC_Os03g10620.1

UniGene: Os.11186

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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