D6PK Antibody

Code CSB-PA176747XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) D6PK Polyclonal antibody
Uniprot No.
Target Names
D6PK
Alternative Names
D6PK antibody; PK64 antibody; At5g55910 antibody; MYN21.2 antibody; Serine/threonine-protein kinase D6PK antibody; EC 2.7.11.1 antibody; D6 protein kinase antibody; Serine/threonine-protein kinase AGC1-1 antibody; Serine/threonine-protein kinase AtPK64 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana D6PK protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein kinase that regulates the auxin transport activity of PIN auxin efflux facilitators by direct phosphorylation. D6PK-mediated PIN phosphorylation promotes auxin transport in the hypocotyl and this is a prerequisite for PHOT1-dependent hypocotyl bending. Phosphorylates PIN1, PIN2, PIN3, PIN4 and PIN7 in vitro and PIN1 in vivo. Acts as a lipid domain-dependent mediator of root epidermal planar polarity via the binding to phosphatidylinositol phosphates (e.g. PtdIns(3)P, PtdIns(4)P, PtdIns(5)P, PtdIns(3,5)P(2), PtdIns(4,5)P(2), PtdIns(3,4,5)P(3) and phosphatidic acid).
Gene References into Functions
  1. the association of D6PK with the basal plasma membrane and PIN-FORMED auxin efflux carriers is dependent on the phospholipid composition of the plasma membrane as well as on the phosphatidylinositol phosphate 5-kinases PIP5K1 and PIP5K2 in epidermis cells of the primary root. D6PK directly binds polyacidic phospholipids through a polybasic lysine-rich motif in the middle domain of the kinase. PMID: 27836964
Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Database Links

KEGG: ath:AT5G55910

STRING: 3702.AT5G55910.1

UniGene: At.23078

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