DA1 Antibody

Code CSB-PA314519XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DA1 Polyclonal antibody
Uniprot No.
Target Names
DA1
Alternative Names
DA1 antibody; SOD1 antibody; At1g19270 antibody; T29M8.14Protein DA1 antibody; Protein SUPPRESSOR OF LARGE SEED AND ORGAN PHENOTYPES OF DA1-1 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin receptor that limits final seed and organ size by restricting the period of cell proliferation. May act maternally to control seed mass. Acts synergistically with DA2 to regulate seed size. Functions synergistically with DA2 to restrict cell proliferation in the maternal integuments of ovules and developing seeds. Functions antagonistically in a common pathway with UBP15 to regulate seed size. Associates physically with UBP15 and modulates the stability of UBP15, which promote cell proliferation in the integuments of ovules and developing seeds. Functions as peptidase and cleaves the N-terminal sequence of E3 ubiquitin-protein ligases BB and DA2 in a ubiquitin-dependent manner. Cleaves the deubiquitinating enzyme UBP15, which promotes cell proliferation, and the transcription factors TCP15 and TCP22, which promote cell proliferation and repress endoreduplication. Involved in the promotion of leaf senescence, in addition to its function in restricting plant growth. Acts redundantly with DAR1 and DAR2 to regulate endoreduplication during leaf development. Together with DAR1 and DAR2, modulates the protein stability of the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes.
Gene References into Functions
  1. DA1 role in controlling leaf growth and senescence PMID: 28003326
  2. DA1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins. PMID: 28167503
  3. Overexpression of a da1-1 cDNA dramatically increases seed and organ size of wild-type plants. PMID: 18483219
Database Links

KEGG: ath:AT1G19270

STRING: 3702.AT1G19270.1

UniGene: At.21826

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