Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dacA Polyclonal antibody

Uniprot No.

P0AEB2

Target Names

dacA

Alternative Names

dacA antibody; pfv antibody; b0632 antibody; JW0627D-alanyl-D-alanine carboxypeptidase DacA antibody; DD-carboxypeptidase antibody; DD-peptidase antibody; EC 3.4.16.4 antibody; Beta-lactamase antibody; EC 3.5.2.6 antibody; Penicillin-binding protein 5 antibody; PBP-5 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) dacA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.

Gene References into Functions

Studied alanine substitution with glutamic acid at Position 184 in Escherichia coli D-alanyl-D-alanine carboxypeptidase (PBP5); results found substitution of A184 to E of Omega-like loop alone can introduce cephalosporinase activity in E. coli PBP5. PMID: 29549627
Authors infer that biochemically DacD and PBP5 are more closely related to each other than to PBP6, enabling DacD and PBP5 to play a nearly similar physiological function in terms of recovering the lost beta-lactam resistance. PMID: 22978571
The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. PMID: 21464593
Results indicating a change in the active-site microarchitectures of PBP5 and 6 as a plausible cause of the difference in their biochemical behaviors. PMID: 21163680
Reversion to beta-lactam resistance was observed in the mutants upon complementing with cloned PBP5, indicating the involvement of PBP5 in maintaining an O-antigen-independent intrinsic beta-lactam resistance in E. coli cells. PMID: 20047819
The roles of the residues Lys47 and Lys213 in the catalytic machinery of PBP 5 was explored. The pH dependence of kcat/Km and of kcat for the wild-type PBP 5 and for the two gamma-thialysine mutant variants at positions 47 and 213 were evaluated. PMID: 17685588

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Subcellular Location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=N-terminal lies in the periplasmic space.

Protein Families

Peptidase S11 family

Database Links

KEGG: ecj:JW0627

STRING: 316385.ECDH10B_0701

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dacA Antibody

Product Details

Customer Reviews and Q&A

Target Background