daf-9 Antibody

Datasheet

Code	CSB-PA785642XA01CXY
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans daf-9 Polyclonal antibody

Uniprot No.

H2KYS3

Target Names

daf-9

Alternative Names

daf-9 antibody; T13C5.1Cytochrome P450 daf-9 antibody; EC 1.14.15.15 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans daf-9 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Converts the 3-keto steroids 4-cholesten-3-one and lathosterone into the carboxylic metabolites 3-keto-4-cholestenate (Delta(4)-dafachronic acid, Delta(4)-DA) and 3-keto-7,(5a)-cholestenate (Delta(7)-dafachronic acid, Delta(7)-DA) respectively, by catalyzing successive oxidations at C-26. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) DAF-12, suppressing dauer formation and inducing reproductive growth. In a non-cell autonomous manner, negatively regulates body wall muscle arm extensions to motor neurons probably by preventing daf-12 isoform b activation. May be involved in thermotolerance.

Gene References into Functions

Expression of DAF-9 is increased in C. elegans subjected to dietary restriction. PMID: 25209682
results support the hypothesis that ncr-1 and ncr-2 are involved in intracellular cholesterol processing in C. elegans, and that a sterol-signaling defect is responsible for the Daf-c phenotype of the ncr-2; ncr-1 mutant PMID: 15509773
We propose that the dauer rescuing activity is a hormone synthesized by DAF-9 that acts through DAF-12. PMID: 15569358
We identified two distinct 3-keto-cholestenoic acid metabolites of DAF-9, a cytochrome P450 involved in hormone production, that function as ligands for DAF-12. PMID: 16529801

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Expressed in the 2 embryonic head hypodermal cells XXXL/R.

Database Links

KEGG: cel:CELE_T13C5.1

STRING: 6239.T13C5.1a

UniGene: Cel.9170

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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