DAR2 Antibody

Code CSB-PA611864XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DAR2 Polyclonal antibody
Uniprot No.
Target Names
DAR2
Alternative Names
DAR2 antibody; LRD3 antibody; At2g39830 antibody; T5I7.13Protein DA1-related 2 antibody; Protein LATERAL ROOT DEVELOPMENT 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DAR2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts redundantly with DA1 and DAR1 to regulate endoreduplication during leaf development. Together with DA1 and DAR1, modulates the protein stability of the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes. Involved in root phloem development. Is an essential component of early phloem development, long-distance delivery of phloem content, and proper maintenance of root system architecture. Involved in the control of root meristem size. Functions genetically downstream of cytokinin and IAA3 to maintain normal auxin distribution by influencing polar auxin transport. Acts through the PLETHORA pathway, upstream of PLT1 and PLT2 to influence root stem cell niche activity and thus control root meristem size.
Gene References into Functions
  1. DA1, DAR1, and DAR2 physically interact with the transcription factors TCP14 and TCP15, which repress endoreduplication. PMID: 25757472
  2. acts downstream of cytokinin and SHY2 but upstream of PLT1/2 to affect root meristem size PMID: 23518585
  3. DAR2 acts through the PLETHORA pathway to influence root stem cell niche activity and therefore control root meristem size. PMID: 23296689
  4. LATERAL ROOT DEVELOPMENT 3 is expressed in the phloem. Mutants display reduced primary root growth, altered root system architecture, and altered phloem callose deposition. PMID: 21749503
Tissue Specificity
Expressed in the vasculature of leaves, inflorescence stems, flowers, hypocotyls, and primary and lateral roots. In roots, expressed in phloem companion cells.
Database Links

KEGG: ath:AT2G39830

STRING: 3702.AT2G39830.1

UniGene: At.37124

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