Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DBF2 Polyclonal antibody

Uniprot No.

P22204

Target Names

DBF2

Alternative Names

DBF2 antibody; YGR092WCell cycle protein kinase DBF2 antibody; EC 2.7.11.1 antibody; Dumbbell forming protein 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DBF2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Ser/Thr-protein kinase involved in the mitotic exit network (MEN) and required after the metaphase to anaphase cell cycle transition. Phosphorylates CHS2 to regulate its dynamics and chitin synthesis at the division site during cytokinesis. Coordinates septin and actomyosin ring (AMR) functions during cytokinesis through the phosphorylation of HOF1. In complex with MOB1, phosphorylates CDC14 at sites adjacent to its nuclear localization sequence, thereby retaining CDC14 in the cytoplasm. Binds also to SWI5 and CLB2 mRNAs cotranscriptionally to regulate their decay. In the nucleus, the DBF2-MOB1 complex regulates passenger protein localization during anaphase. Mediates sorbic acid stress tolerance through promoting vacuolar H(+)-ATPase function, probably through phosphorylation of VMA1 and VMA2 subunits.

Gene References into Functions

y usage of an enhanced system for sporulation-induced protein depletion, we demonstrate that the core mitotic exit network (MEN) is involved in age-based SPB selection. Moreover, efficient genome inheritance requires Dbf2/20-Mob1 during a late step in spore maturation. PMID: 28450458
Dbf2 coordinates septin and actomyosin ring functions during cytokinesis through the regulation/control of Hof1. PMID: 23447700
Dbf2 regulates primary septum formation through at least two independent pathways PMID: 22573892
Data show that polo-like kinase Cdc5 first phosphorylates Hof1 to allow subsequent phosphorylation by Dbf2-Mob1. PMID: 21498574
required for establishing passenger protein localization on the mitotic spindle PMID: 16176976

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Bud neck. Nucleus. Note=Localizes on spindle ole body (SPB) for much of the cell cycle and migrates from there to the bud neck in late mitosis. SPB localization during anaphase coincides with activation kinase activity and depends on TEM1 and CDC15. In absence of BUB2, localizes to SPB in cell cycle stages other than anaphase and telophase.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: sce:YGR092W

STRING: 4932.YGR092W

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DBF2 Antibody

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