hupA Antibody

1. The cellular HUalpha content is proportional to the cell size, whereas its concentration is size independent. PMID: 28961819
2. By performing ChIP-seq experiments of each subunit of HU in the absence of the other subunit, genome-wide maps of DNA binding of the proteins in their hetero- and homodimeric forms were defined. PMID: 22180530
3. Using the E. coli K-12/HUalpha(E38K, V42L) variant as a model, the authors propose that traditional commensal E. coli can adopt an invasive lifestyle through reprogramming its cellular transcription, without gross genetic changes. PMID: 21896677
4. It is remarkable that the observed salt concentration dependency of the binding constants of the three DNA binding modes of the HUalpha subunit is similar, given the large differences in the binding site sizes between these modes. PMID: 21513716
5. Under physiological conditions, the three possible dimeric forms of the E. coli HU protein (EcHUalpha, EcHUbeta, and EcHUalphabeta) are in thermal equilibrium between two dimeric conformations (N<--> I) varying in their secondary structure content. PMID: 20936276
6. Unlike the dimeric wild-type HU, HUalpha(E38K,V42L) is an octamer and wraps DNA around its surface; formation of wrapped nucleoprotein complexes by HUalpha(E38K,V42L) leads to a high degree of DNA condensation. PMID: 17062578
7. An HU dimer requires 24 nt of ssDNA for initial binding, and 12 nt of ssDNA for each additional dimer binding. PMID: 18096614
8. HU(alphabeta) interacts with duplex DNA using three different nonspecific binding modes PMID: 18657548

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KEGG: ecj:JW3964

STRING: 316385.ECDH10B_4189