DBP5 Antibody

Code CSB-PA323231XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DBP5 Polyclonal antibody
Uniprot No.
Target Names
DBP5
Alternative Names
DBP5 antibody; RAT8 antibody; YOR046CATP-dependent RNA helicase DBP5 antibody; EC 3.6.4.13 antibody; DEAD box protein 5 antibody; Helicase CA5/6 antibody; Ribonucleic acid-trafficking protein 8 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DBP5 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. Contributes to the blocking of bulk poly(A)+ mRNA export in ethanol-stressed cells. May also be involved in early transcription.
Gene References into Functions
  1. Dbp5 supports ribosomal transport by capturing ribosomal subunits upon their cytoplasmic appearance at the nuclear pore complex, possibly by binding export factors such as Mex67. PMID: 26872259
  2. These data show that ATP binds Dbp5 weakly in rapid equilibrium with a binding affinity (KT~4 mM) comparable to the KM for steady-state cycling, while ADP binds an order of magnitude more tightly (KD~0.4 mM). PMID: 26730886
  3. changes in the localization of Rat8p contribute to the selective export of mRNA in ethanol stressed cells PMID: 15280434
  4. the Nup159 NTD functions in mRNA export as a binding platform, tethering shuttling Dbp5 molecules at the nuclear periphery PMID: 15574330
  5. DEAD-box ATPase Dbp5 is specifically required to displace Mex67p from exported messenger ribonucleoproteins, thus terminating export. PMID: 16307927
  6. role in translation termination; it is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes PMID: 17272721
  7. ADP-bound form may perform an important function in displacing export factors from mRNA PMID: 18397738
  8. Wild-type Rat8p localizes to NPCs and diffusely throughout the cell but rat8-2p localized to cytoplasmic granules at nonpermissive temperature that are distinct from P-bodies PMID: 18689878

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Subcellular Location
Cytoplasm. Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Nuclear pore complex cytoplasmic fibrils. Accumulates in the nucleus rapidly and reversibly in response to ethanol stress.
Protein Families
DEAD box helicase family, DDX19/DBP5 subfamily
Database Links

KEGG: sce:YOR046C

STRING: 4932.YOR046C

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