Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DCL1 Polyclonal antibody

Uniprot No.

Q9SP32

Target Names

DCL1

Alternative Names

DCL1 antibody; ASU1 antibody; CAF antibody; SIN1 antibody; SUS1 antibody; At1g01040 antibody; T25K16.4Endoribonuclease Dicer homolog 1 antibody; EC 3.1.26.- antibody; Dicer-like protein 1 antibody; AtDCL1 antibody; Protein ABNORMAL SUSPENSOR 1 antibody; Protein CARPEL FACTORY antibody; Protein SHORT INTEGUMENTS 1 antibody; Protein SUSPENSOR 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DCL1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.

Gene References into Functions

DCL1 interacts with PIF4 which integrates miRNA biogenesis and red light signaling by regulating the transcription of a group of miRNA genes and the stability of DCL1 during dark-to-red-light or red-light-to-dark transitions. PMID: 29522510
a detailed modeling and molecular dynamics study of dsRNA recognition by DCL1-1. PMID: 26987516
CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. PMID: 26473486
The first double stranded RNA binding domain (dsRBD) of DCL1 is intrinsically disordered, but it can still perform its expected role, binding dsRNA and working jointly with the second dsRBD in the recognition of the substrate. PMID: 26101256
DCL1 complexes canonically cut pri-miRNAs at a distance of 16-17 bp from a reference single-stranded loop region. DCL1 also adjusts processing sites toward an internal loop through its helicase domain. PMID: 23934148
we obtained the solution structure of DCL1-B and showed that it has a canonical fold with some variations that can be functionally relevant. We found that this domain binds precursor RNA with no preference for different regions PMID: 23194006
The DCL1 helicase domain attenuates cleavage activity and is required for the accurate processing of primary miRNA. PMID: 22474216
DCL1 is required for cell differentiation events as early as the eight-cell stage and soon thereafter for proper division of the hypophysis and subprotoderm cells PMID: 21123653
DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation. PMID: 20870966
RNA interference of DCL1 and AGO1 led to dominant negative phenotypes and decreased accumulation of several small rna tested in the transgenic resynthesized allotetraploids. PMID: 20409179
These data suggest that P1/HC-Pro defects in development do not result from general impairments in small RNA pathways and raise the possibility that DCL1 participates in processes in addition to miRNA biogenesis. PMID: 16214897

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Subcellular Location

Nucleus. Note=Localizes to nuclear dicing body (also named D body), a nuclear body distributed throughout the nucleoplasm and involved in miRNA processing.

Protein Families

Helicase family, Dicer subfamily

Tissue Specificity

Highly expressed in flowers and seeds and detected in leaves and stems. Found in ovule integuments, inflorescence and floral meristems, stigma of flowers until just before pollination, vasculature of the funiculus, and embryo.

Database Links

KEGG: ath:AT1G01040

STRING: 3702.AT1G01040.2

UniGene: At.22088

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DCL1 Antibody

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