Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 1 (DCL1), partial

Code CSB-YP890400DOA
MSDS
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Source Yeast
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Code CSB-EP890400DOA
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Source E.coli
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Code CSB-EP890400DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890400DOA
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Source Baculovirus
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Code CSB-MP890400DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DCL1
Uniprot No.
Alternative Names
DCL1; ASU1; CAF; SIN1; SUS1; At1g01040; T25K16.4Endoribonuclease Dicer homolog 1; EC 3.1.26.-; Dicer-like protein 1; AtDCL1; Protein ABNORMAL SUSPENSOR 1; Protein CARPEL FACTORY; Protein SHORT INTEGUMENTS 1; Protein SUSPENSOR 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.
Gene References into Functions
  1. DCL1 interacts with PIF4 which integrates miRNA biogenesis and red light signaling by regulating the transcription of a group of miRNA genes and the stability of DCL1 during dark-to-red-light or red-light-to-dark transitions. PMID: 29522510
  2. a detailed modeling and molecular dynamics study of dsRNA recognition by DCL1-1. PMID: 26987516
  3. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. PMID: 26473486
  4. The first double stranded RNA binding domain (dsRBD) of DCL1 is intrinsically disordered, but it can still perform its expected role, binding dsRNA and working jointly with the second dsRBD in the recognition of the substrate. PMID: 26101256
  5. DCL1 complexes canonically cut pri-miRNAs at a distance of 16-17 bp from a reference single-stranded loop region. DCL1 also adjusts processing sites toward an internal loop through its helicase domain. PMID: 23934148
  6. we obtained the solution structure of DCL1-B and showed that it has a canonical fold with some variations that can be functionally relevant. We found that this domain binds precursor RNA with no preference for different regions PMID: 23194006
  7. The DCL1 helicase domain attenuates cleavage activity and is required for the accurate processing of primary miRNA. PMID: 22474216
  8. DCL1 is required for cell differentiation events as early as the eight-cell stage and soon thereafter for proper division of the hypophysis and subprotoderm cells PMID: 21123653
  9. DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation. PMID: 20870966
  10. RNA interference of DCL1 and AGO1 led to dominant negative phenotypes and decreased accumulation of several small rna tested in the transgenic resynthesized allotetraploids. PMID: 20409179
  11. These data suggest that P1/HC-Pro defects in development do not result from general impairments in small RNA pathways and raise the possibility that DCL1 participates in processes in addition to miRNA biogenesis. PMID: 16214897

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Subcellular Location
Nucleus. Note=Localizes to nuclear dicing body (also named D body), a nuclear body distributed throughout the nucleoplasm and involved in miRNA processing.
Protein Families
Helicase family, Dicer subfamily
Tissue Specificity
Highly expressed in flowers and seeds and detected in leaves and stems. Found in ovule integuments, inflorescence and floral meristems, stigma of flowers until just before pollination, vasculature of the funiculus, and embryo.
Database Links

KEGG: ath:AT1G01040

STRING: 3702.AT1G01040.2

UniGene: At.22088

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