Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) At3g03300 Polyclonal antibody

Uniprot No.

Q3EBC8

Target Names

At3g03300

Alternative Names

At3g03300 antibody; T17B22.1Endoribonuclease Dicer homolog 2 antibody; EC 3.1.26.- antibody; Dicer-like protein 2 antibody; AtDCL2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana At3g03300 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of natural small interfering RNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving small dsRNAs into 24 nucleotide nat-siRNAs. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL4 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs (ra-siRNAs) which derive from heterochromatin and DNA repeats such as transposons. Plays a role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the turnip crinkle virus (TCV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL2 function in RNA silencing. Does not seem to be involved in microRNAs (miRNAs) processing.

Gene References into Functions

DCL2 is crucial for RDR6-dependent systemic Posttranscriptional gene silencing. PMID: 28928141
DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation PMID: 28267232
DCL2/4-dependent and -independent RNA silencing pathways are involved, and may crosstalk with basal innate immunity pathways, in host defense and in cross-protection. PMID: 24804808
AGO2 and HEN1 participate in the DCL2-mediated antiviral defense to ensure the survival of Turnip crinkle virus-infected plants at high temperature. PMID: 22496240
Data revealed that AtDCL2 is also preferentially expressed in the Arabidopsis egg cell. PMID: 21673515
DCL4 and DCL2 exhibit specific, hierarchical antiviral activities; inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2 PMID: 16741077
DCL2 antagonizes the production of miRNAs and siRNAs by DCL1 in certain circumstances and can substitute for DCL4 when DCL4 activity is reduced or inhibited by viruses. PMID: 16810317
Study shows that unidirectional (3-->5') transitivity requires the hierarchical and redundant functions of DCL4 and DCL2 acting downstream from RDR6 to produce 21- and 22-nt-long siRNAs, respectively. PMID: 17592042
DCL2 plays an essential, as opposed to redundant, role in transitive silencing of transgenes and may play a more important role in silencing of viruses than currently thought PMID: 18335032
Data describe three alternative splicing (AS) forms of AtDCL2: one with both intron 8 and intron 10 are retained, second with only intron 8 is retained and third with no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. PMID: 19011859
The results reveal that dcl2, dcl3 and dcl4 mutations similarly affect post-transcriptional gene silencing (PTGS) mediated by a sense transgene and PTGS mediated by inverted repeats. PMID: 19343050
Cross-protection by Fny-CMV2b against Fny-CMV infection was not abolished in dcl2, or dcl4 mutant plants. PMID: 19474248
We have demonstrated that DCLs 2, 3 and 4 are dispensable for Salicylic acid-induced resistance to Tobacco Mosaic Virus and Cucumber Mosaic Virus. PMID: 19710258

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Helicase family, Dicer subfamily

Database Links

KEGG: ath:AT3G03300

STRING: 3702.AT3G03300.1

UniGene: At.43488

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At3g03300 Antibody

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