Dcr-1 Antibody

Code CSB-PA893259XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Dcr-1 Polyclonal antibody
Uniprot No.
Target Names
Dcr-1
Alternative Names
Dcr-1 antibody; CG4792Endoribonuclease Dcr-1 antibody; Protein dicer-1 antibody; EC 3.1.26.- antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Dcr-1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential for RNA interference (RNAi); double-stranded RNA (dsRNA) induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger. May carry out the initiation step of RNAi by cleaving dsRNA to produce 22 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. During embryogenesis, involved in germline fate determination.
Gene References into Functions
  1. [review] Dicer-1 lacks a functional DExD/H helicase domain and is responsible for processing hairpin-structured pre-microRNAs. PMID: 24556400
  2. Dicer-1 is required for siRNA-directed mRNA cleavage. Dicer-1 facilitates distinct steps in the assembly of siRISC, and is essential for miRISC-directed translation repression. PMID: 15066283
  3. Dcr-1 is critical for assembly of microRNA as shown in dcr-1 knockout egg extract PMID: 17928574
  4. These results reveal a novel interaction between Flock house virus B2 protein and Dicer that leads to suppression of siRNA biogenesis. PMID: 19193719
Protein Families
Helicase family, Dicer subfamily
Database Links

KEGG: dme:Dmel_CG4792

STRING: 7227.FBpp0083717

UniGene: Dm.1283

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