dcr1 Antibody

Code CSB-PA600673XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) dcr1 Polyclonal antibody
Uniprot No.
Target Names
dcr1
Alternative Names
dcr1 antibody; SPCC188.13c antibody; SPCC584.10cProtein Dicer antibody; Cell cycle control protein dcr1 antibody; RNA interference pathway protein dcr1) [Includes: Endoribonuclease dcr1 antibody; EC 3.1.26.-); ATP-dependent helicase dcr1 antibody; EC 3.6.4.-)] antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) dcr1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2.; Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Digests double-stranded RNA (dsRNA) producing 21 to 23 bp dsRNAs, so-called interfering RNAs (siRNA). Required for both post-transcriptional and transcriptional gene silencing. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3 'Lys-10' methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat.
Gene References into Functions
  1. Findings suggest that in S. pombe Dcr1 has a genome-wide role in terminating transcription by releasing Pol II at sites of collision between transcription and replication and thus maintains genome stability. PMID: 25417108
  2. Studies indicate that S. pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). PMID: 24462781
  3. Data indicate that Argonaute, loaded with longer RNA precursors, recruits Triman (SPBC29A10.09c, 3'-5' Exonuclease) to generate dicer-independent priRNAs mature priRNAs and siRNAs. PMID: 24095277
  4. these results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleocytoplasmic trafficking and not substrate binding PMID: 21847092
  5. Dcr1 has a role in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe [review] PMID: 17981703

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Helicase family, Dicer subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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