dctA Antibody

Datasheet

Code	CSB-PA364102XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dctA Polyclonal antibody

Uniprot No.

P0A830

Target Names

dctA

Alternative Names

dctA antibody; b3528 antibody; JW3496 antibody; Aerobic C4-dicarboxylate transport protein antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) dctA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Responsible for the aerobic transport of the dicarboxylates fumarate, L- and D-malate and to a lesser extent succinate, from the periplasm across the inner membrane.

Gene References into Functions

The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models. PMID: 29203472
DcuS is required for location of DctA and DcuR at the poles and formation of tripartite DctA/DcuS/DcuR sensor/regulator complexes. PMID: 25549248
C4-dicarboxylate sensing by DctA/DcuS resides in DcuS, and that DctA serves as an activity switch. PMID: 25135747
Reorganizing the DcuS homodimer and its interaction with DctA activate the kinase. PMID: 24039243
It is concluded that DctA forms a functional unit or sensor complex with DcuS through specific interaction sites. PMID: 22780562

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Database Links

KEGG: ecj:JW3496

STRING: 316407.85676516

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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dctA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background