DDB1A Antibody

Code CSB-PA868099XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DDB1A Polyclonal antibody
Uniprot No.
Target Names
DDB1A
Alternative Names
DDB1A antibody; At4g05420 antibody; C6L9.100DNA damage-binding protein 1a antibody; UV-damaged DNA-binding protein 1a antibody; DDB1a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DDB1A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of light signal transduction machinery. Involved in repression of photomorphogenesis in darkness by participating in the CDD complex, a complex probably required to regulate the activity of ubiquitin conjugating enzymes (E2s). Repression of photomorphogenesis is probably mediated by ubiquitination and subsequent degradation of photomorphogenesis-promoting factors such as HY5, HYH and LAF1. Plays a role in DNA repair by forming with DDB2 the UV-damaged DNA-binding protein complex (UV-DDB). Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex.
Gene References into Functions
  1. ABD1 interacts with DDB1 in vitro and in vivo, indicating that it likely functions as a CUL4 E3 ligase substrate receptor. PMID: 24563203
  2. that a DDB1(WDR55) complex could regulate embryo and endosperm development. PMID: 23803743
  3. UVH6, DDB1A, and DDB2 all contribute to UV tolerance, heat tolerance and floral patterning. PMID: 24157211
  4. DDB1A and DDB1B contribute to DET1 regulation of adult plant growth. PMID: 23450167
  5. DDB1a is an novel RUB-modified protein. PMID: 22893627
  6. CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting. PMID: 21240189
  7. data suggest that the plant CUL4-DDB1A(CSAat1A and B) complex represents a unique mechanism to promote ubiquitination of substrates in response to DNA damage PMID: 20622147
  8. demonstrated that DDB1a, like DDB1b, is critical for embryo development, and that both proteins have distinct functions in whole plant development PMID: 20499085
  9. DDB1A-3HA overexpression affected phenotypes in a variety of DET1 backgrounds, reduced epitope-tagged DET1 levels, and, correlatively, in general dampened the rescue of det1 mutants by the DET1-DDB1A complex. PMID: 19921247
  10. Arabidopsis cullin 4 is shown to assemble with DDB1, RBX1, DET1 and DDB2 in vitro and in planta.[DDB1A] PMID: 16792691
  11. DDB1A-dependent phenotypes indicate a competition between DDB2- and DET1-containing complexes for available DDB1A, while, for DDB1A-independent phenotypes, DDB1B is able to fulfill this role. PMID: 17409070
  12. CUL4-DDB1A-DDB2 complex is involved in global genome repair of UV-induced DNA lesions. PMID: 18551167
  13. Results show that DDB1A is required for UV regulation of DDB1B and DDB2 mRNA levels while DDB1A is sufficient to increase Arabidopsis UV tolerance. PMID: 19288212

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
DDB1 family
Database Links

KEGG: ath:AT4G05420

STRING: 3702.AT4G05420.1

UniGene: At.32663

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