Rm62 Antibody

Code CSB-PA322496XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Rm62 Polyclonal antibody
Uniprot No.
Target Names
Rm62
Alternative Names
Rm62 antibody; Dmp68 antibody; p62 antibody; CG10279 antibody; ATP-dependent RNA helicase p62 antibody; EC 3.6.4.13 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Rm62 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, rRNA processing and miRNA processing, as well as transcription regulation. Plays a role in innate immunity. Specifically restricts bunyavirus infection, including Rift Valley fever virus (RVFV) or La Crosse virus (LACV), but not vesicular stomatitis virus (VSV), in an interferon- and DROSHA-independent manner.
Gene References into Functions
  1. Through an RNAi screen in Drosophila cells, study identified Rm62 as an anti-RVFV helicase gene. Rm62 is also essential for resistance to RVFV infection in vivo, as RVFV-challenged Rm62-deficient flies showed increased viral replication and mortality. PMID: 25126784
  2. RNA helicase interacts with the well-characterized histone methyltransferase SU(VAR)3-9 via its N-terminus, which provides a potential mechanism for the targeting of H3K9 methylation to highly regulated genes. PMID: 21674064
  3. Rm62, a DEAD-box RNA helicase, complexes with DSP1 in Drosophila embryos PMID: 20196121
Subcellular Location
Nucleus. Nucleus, nucleolus. Cytoplasm, cytosol.
Protein Families
DEAD box helicase family, DDX5/DBP2 subfamily
Database Links

KEGG: dme:Dmel_CG10279

STRING: 7227.FBpp0078301

UniGene: Dm.1520

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