laf-1 Antibody

Code CSB-PA130795XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans laf-1 Polyclonal antibody
Uniprot No.
Target Names
laf-1
Alternative Names
laf-1 antibody; Y71H2AM.19ATP-dependent RNA helicase laf-1 antibody; EC 3.6.4.13 antibody; DEAD-box RNA helicase laf-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans laf-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Multifunctional ATP-dependent RNA helicase. Plays a role in RNA remodeling, but is not required for RNA unwinding. Binds to RNA in a concentration-dependent manner to stimulate annealing between two complementary strands of RNA. This process is also dependent upon ATP; ATP reduces binding to RNA and subsequently diminishes RNA annealing. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. Involved in the regulation of transcription and translation initiation. Involved in innate immunity. Involved in both stress and inflammatory responses. Promotes liquid-liquid phase separation of P granules, which is a process important for intracellular organization and stress granule assembly. Required for embryonic development. Plays a role in sexual cell fate determination by negatively regulating the translation of the sex determining protein tra-2. May play a protective role in the response to heat and oxidative stress. May negatively regulate extrinsic apoptotic signaling pathway via death domain receptors. May be involved in mitotic chromosome segregation.
Gene References into Functions
  1. The article describes RNA remodeling activity of human DDX3X and Caenorhabditis elegans LAF-1 tuned by protein concentration, RNA length, and ATP. PMID: 27546789
Subcellular Location
Cytoplasm. Cytoplasmic granule. Nucleus. Cytoplasm, Stress granule. Inflammasome. Cell membrane. Cell projection, lamellipodium.
Protein Families
DEAD box helicase family, DDX3/DED1 subfamily
Tissue Specificity
Expressed in the germline and soma of young adult hermaphrodites.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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