degP Antibody

Code CSB-PA366225XA01EOD
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 degP Polyclonal antibody
Uniprot No.
Target Names
degP
Alternative Names
degP antibody; htrA antibody; ptd antibody; Z0173 antibody; ECs0165Periplasmic serine endoprotease DegP antibody; EC 3.4.21.107 antibody; Heat shock protein DegP antibody; Protease Do antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 degP protein
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).
Subcellular Location
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Peptidase S1C family
Database Links

KEGG: ece:Z0173

STRING: 155864.Z0173

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