degS Antibody

Code CSB-PA365133XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) degS Polyclonal antibody
Uniprot No.
Target Names
degS
Alternative Names
degS antibody; hhoB antibody; htrH antibody; b3235 antibody; JW3204 antibody; Serine endoprotease DegS antibody; EC 3.4.21.107 antibody; Site-1 protease DegS antibody; S1P protease DegS antibody; Site-1-type intramembrane protease antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) degS protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
A site-1 protease (S1P) that cleaves the peptide bond between 'Val-148' and 'Ser-149' in RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMP) and then initiates RseA (anti sigma-E factor) degradation by cleaving its periplasmic domain, making it a substrate for subsequent cleavage by RseP. This cascade ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly. Required for basal and stress-induced degradation of RseA.
Gene References into Functions
  1. Mechanisms for allosteric activation of protease DegS by ligand binding and oligomerization has been described. PMID: 27556733
  2. analysis of allosteric regulation of DegS protease subunits through a shared energy landscape PMID: 23201899
  3. Allostery is an intrinsic property of the protease domain of DegS PMID: 20739286
  4. crystal structure of DegS PMID: 15272167
  5. Residues involved in the DegS allosteric switch are conserved in the DegP/HtrA and HtrA2/Omi families, suggesting that many PDZ proteases use a common mechanism of allosteric activation. PMID: 17981123
  6. Self-cleavage occurs when HtrA reassembles back into the hexameric structure PMID: 19103920

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Subcellular Location
Cell inner membrane; Single-pass membrane protein.
Protein Families
Peptidase S1C family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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