Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DEK1 Polyclonal antibody

Uniprot No.

Q8RVL2

Target Names

DEK1

Alternative Names

DEK1 antibody; EMB1275 antibody; EMB80 antibody; At1g55350 antibody; F7A10.23Calpain-type cysteine protease DEK1 antibody; EC 3.4.22.- antibody; Phytocalpain DEK1 antibody; Protein DEFECTIVE KERNEL 1 antibody; AtDEK1 antibody; Protein EMBRYO DEFECTIVE 1275 antibody; Protein EMBRYO DEFECTIVE 80 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DEK1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential protease involved in epiderm development. Required for aleurone cell development in the endosperm probably by maintaining and restricting the aleurone and embryonic epidermal L1 cell-layer fates as well as meristems organization. Involved in the maintenance of adaxial/abaxial axis information in developing leaves, probably by regulating cell proliferation and expansion. Does not need calcium ions to be active. Required for the formation of giant cells in sepals by determining cell fate and promoting endoreplication.

Gene References into Functions

Cell wall related genes are regulated by a feedback mechanism in response to DEK1-induced changes in the cell wall, and contribute to the phenotypes seen in plants with altered DEK1 expression. PMID: 28692330
DEK1 which is essential for development beyond early embryogenesis, is associated with a mechanically activated Ca(2+) current. PMID: 29044106
a novel role for DEK1 in the regulation of leaf epidermal cell wall structure, is reported. PMID: 27756823
A role for DEK1 in regulating microtubule-orchestrated cell wall orientation during cell division can explain its effects on embryonic development, and suggests a more general function for calpains in microtubule organization in eukaryotic cells. PMID: 26220906
DEK1 influences HD-ZIP IV gene expression, and thus epidermis differentiation, by promoting cell adhesion and communication in the epidermis. PMID: 25953348
A specific role for AtDEK1 during embryogenesis and the potential key importance of L1 specification at the globular stage for subsequent progression through embryogenesis are reported. PMID: 16167900
Data suggest a model for aleurone cell fate specification in which DEK1 perceives and/or transmits a positional signal PMID: 17933905
DEK1 has a role in growth regulation. PMID: 18952779

Hide All

Subcellular Location

Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm.

Protein Families

Peptidase C2 family

Tissue Specificity

Mostly expressed in meristems and organ primordia. Expressed at low levels in young and germinating seeds at 10 ppm and in seedling roots at 67 ppm. Present in most tissues at a low level.

Database Links

KEGG: ath:AT1G55350

STRING: 3702.AT1G55350.5

UniGene: At.17726

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA695640XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

DEK1 Antibody

Product Details

Customer Reviews and Q&A

Target Background