Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DGAT1 Polyclonal antibody

Uniprot No.

Q9SLD2

Target Names

DGAT1

Alternative Names

DGAT1 antibody; ABX45 antibody; DAGAT antibody; TAG1 antibody; At2g19450 antibody; F3P11.5Diacylglycerol O-acyltransferase 1 antibody; AtDGAT1 antibody; EC 2.3.1.20 antibody; Protein TRIACYLGLYCEROL 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DGAT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.

Gene References into Functions

DGAT1 expression is regulated by MYB96 in the Arabidopsis seeds. PMID: 29660088
The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 1873%) compared with wild-type seeds. PMID: 29874815
This study identifies the first two regulatory genes, WRI1 and DGAT1, that control the synthesis of all tocochromanol forms in seeds, and shows the existence of a metabolic trade-off between lipid and tocochromanol metabolisms. PMID: 29105089
The results suggest that dedifferentiation is associated with Tag1 activation and that CMT3 rather than DDM1 plays a central role in restraining Tag1 activation via inducing gene body DNA methylation. PMID: 27475038
ABI4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress. PMID: 23942253
Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2. PMID: 22233193
ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency. PMID: 21515696
PDAT1 and DGAT1 have overlapping functions for triacylglycerol synthesis in both seed and pollen, and that the absence of their function leads not only to a reduction in TAG, but also to critical defects in normal pollen and embryo development. PMID: 20040537

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Subcellular Location

Plastid, chloroplast membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily

Tissue Specificity

Ubiquitous. Highest expression in young developing seeds.

Database Links

KEGG: ath:AT2G19450

STRING: 3702.AT2G19450.1

UniGene: At.20259

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DGAT1 Antibody

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