DGD1 Antibody

Code CSB-PA874347XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DGD1 Polyclonal antibody
Uniprot No.
Target Names
DGD1
Alternative Names
DGD1 antibody; At3g11670 antibody; T19F11.7 antibody; Digalactosyldiacylglycerol synthase 1 antibody; chloroplastic antibody; EC 2.4.1.241 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DGD1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Involved in the synthesis of diacylglycerol galactolipids that are specifically found in thylakoid membranes. Specific for alpha-glycosidic linkages. Responsible for the final assembly of galactolipids in photosynthetic membranes. Digalactosyldiacylglycerol (DGDG) provides stability to the photosystem I (PSI) complex, especially to the PsaA, PsaB, PsaC, PsaL and PsaH subunits.
Gene References into Functions
  1. Different diglycosyllipid synthases (DGD1, DGD2, and Chloroflexus glucosyltransferase) were introduced into the dgd1-1 mutant of Arabidopsis in fusion with N-terminal extensions (NDGD1 and NDGD2) targeting to the outer envelope.NDGD1 binds to phosphatidic acid (PA) in membranes and mediates PA-dependent membrane fusion in vitro. PMID: 27601658
  2. At elevated temperatures MC540 is extruded from the dgd1 membrane bilayer around 35 degrees C, whereas in WT, it remains lipid-bound up to 45 degrees C and dgd1 and WT membranes become leaky around 35 and 45 degrees C, respectively. PMID: 20645128
  3. DGDG is important for the stability of the PSI complex. PMID: 15961080
  4. Data show that cold acclimation of the Arabidopsis dgd1 mutant results in recovery from photosystem I-limited photosynthesis. PMID: 16930596
  5. The affected gene in atts02 was identified by positional cloning and encodes digalactosyldiacylglycerol synthase 1 (DGD1). PMID: 17080965
  6. The roles of DGD1 and DGD1 SUPPRESSOR 1 (DGS1) in the expression of nuclear genes encoding enzymes of the alternative galactoglycerolipid pathway are reported.[DGS1] PMID: 18208519

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Subcellular Location
Plastid, chloroplast outer membrane.
Protein Families
Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
Database Links

KEGG: ath:AT3G11670

STRING: 3702.AT3G11670.1

UniGene: At.21419

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