DHAR3 Antibody

Code CSB-PA963888XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DHAR3 Polyclonal antibody
Uniprot No.
Target Names
DHAR3
Alternative Names
DHAR3 antibody; At5g16710 antibody; F5E19.50 antibody; Glutathione S-transferase DHAR3 antibody; chloroplastic antibody; EC 2.5.1.18 antibody; Chloride intracellular channel homolog 3 antibody; CLIC homolog 3 antibody; Glutathione-dependent dehydroascorbate reductase 3 antibody; AtDHAR3 antibody; ChlDHAR antibody; GSH-dependent dehydroascorbate reductase 3 antibody; EC 1.8.5.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DHAR3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses.
Gene References into Functions
  1. The data suggest that (1) DHAR can be decreased to negligible levels without marked effects on ascorbate pools, (2) the cytosolic isoforms are particularly important in coupling intracellular hydrogen peroxide metabolism to glutathione oxidation, and (3) DHAR-dependent glutathione oxidation influences redox-driven salicylic acid accumulation. PMID: 28381499
  2. The findings indicate that DHAR3 regulates both ascorbate and glutathione redox states to acclimate to high intensity light. PMID: 26927949
Subcellular Location
Plastid, chloroplast stroma.
Protein Families
GST superfamily, DHAR family
Database Links

KEGG: ath:AT5G16710

STRING: 3702.AT5G16710.1

UniGene: At.16881

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