D Antibody

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Code	CSB-PA635527XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) D Polyclonal antibody

Uniprot No.

Q24533

Target Names

Alternative Names

D antibody; fish antibody; Sox70D antibody; CG5893SOX domain-containing protein dichaete antibody; Protein fish-hook antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) D protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Essential for segmentation and CNS development. May modulate the actions of other transcription factors, including gap and pair-rule proteins.

Gene References into Functions

Analysis of the functional relationship between SoxNeuro and Dichaete uncovers evidence for redundant and independent functions for each protein, along with unexpected examples of compensation and interdependency. PMID: 24886562
Dichaete acts as a transcriptional hub, controlling multiple regulatory pathways during CNS development. PMID: 24314314
the early neurectoderm we implicate Dichaete in the lateral inhibition pathway and show that Dichaete acts to repress the proneural gene asense PMID: 23289785
These results suggest noncell autonomous functions of Dichaete in projection neuron differentiation as well as a potential role for Dichaete-expressing local interneurons PMID: 22648855
Data show that Dichaete appears to be a novel phosphorylation substrate for Pelle and may function in a Pelle-dependent signaling pathway during oogenesis. PMID: 20563992
Evidence for differential and redundant function of the Sox genes during CNS development in Drosophila PMID: 12183374
Results show that expression of Dichaete protein is detected in the developing oocyte during early to mid stages of oogenesis, exhibits cytoplasmic distribution, and plays a direct post-transcriptional role in regulating RNA functions. PMID: 16894603
The expression of svb in a discreet domain is resolved at the level of SoxN and Dichaete. PMID: 17611224

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Subcellular Location

Nucleus.

Tissue Specificity

Initially expressed in a pair-rule-like pattern which is rapidly replaced by strong neuroectoderm expression.

Database Links

KEGG: dme:Dmel_CG5893

STRING: 7227.FBpp0075488

UniGene: Dm.1835

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D Antibody

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